PJB-2020-256
EVALUATION OF ANTIOXIDANT AND LARVICIDAL ACTIVITIES OF SELECTED MEDICINAL PLANTS AGAINST THE SOUTHREN HOUSE MOSQUITO “CULEX QUINQUEFASCIATUS (SAY)”
Izhar Ahmad
Abstract
The present study was aimed to evaluate Tamarix baluchistanica (Qaiser), Tamarix androssowii (Bunge) and Tamarix mascatensis (Bunge) for their efficacy in DPPH, ABTS free radical scavenging and larvicidal potential against mosquito Cx. quinquefasciatus; an important vector for lymphatic filariasis. The aerial parts of plants dried in shade, extracted with methanol and subsequently evaluated for antioxidant and larvicidal activities. The most active methanolic extract of T. baluchistanica was then subjected to fractionation with different solvents with increasing polarity. Antioxidant bioassays revealed that ethyl acetate fraction was most potent among all the fractions, exhibited IC50 of 32.46 µg/ml and IC90 of 91.32 µg/ml against DPPH radicals, similar to that of ascorbic acid, the reference antioxidant compound used that showed IC50 of 32.48 µg/ml and IC90 of 86.73 µg/ml. Antioxidant activity against ABTS+ cations demonstrated that among all the fractions, least IC50 of 37.94 µg/ml and IC90 of 129.85 µg/ml was also recorded for ethyl acetate fraction of T. baluchistanica methanolic extract, comparable to reference antioxidant, BHT having IC50 of 36.78 µg/ml and IC90 of 113.07 µg/ml . The methanolic extract of T. baluchistanica showed more larvicidal assay against the 3rd instar larvae of mosquito: Cx. quinquefasciatus . The chloroform fractions showed lowest LC50 of 0.06 mg/ml, followed by n-hexane fraction having LC50 of 1.26 mg/ml, while lowest LC90 (2.12 mg/ml) was exhibited by n-hexane fraction, followed by chloroform fraction (7.94 mg/ml). The antioxidant and larvicidal activity of these species are reported for the first time. Further research is required to isolate and identify the active components of the plant extract for effective implication in the control of the vector
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