PJB-2020-252
EFFECT OF GAMMA IRRADIATION ON MOLECULAR CHANGES FOR SOME GENOTYPES OF NIGELLA SATIVA L.
Ahmed Fayez Khafaga
Abstract
To detect RAPD-DNA markers for four Nigella sativa cultivars treated with different doses of gamma radiations, we used seven RAPD primers. Three doses; 0 Gy (as control treatment), 20 Gy and 40 Gy were used. Each genotype revealed different responses on the molecular level to gamma irradiation. The total number of polymorphic fragments (PF) and total specific markers (TSM) ranged from 11 fragments as least value for Elmenia cultivar to 37 fragments as highest value for Turkey1 cultivar. All the total specific markers (TSM) were positive (appeared bands) and negative (disappeared bands) markers in all cultivars with most of the seven arbitrary primers used.
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