PJB-2020-245
Temporal and spatial expression patterns and protein structure prediction of the CMO gene of Hippophae rhamnoides under drought stress
Jiawei Dong
Abstract
Abstract In recent years, drought stress caused by global warming and an uneven distribution of water resources has become one of the most common stress factors in nature; thus, it is of great importance to study the structure and function of plant stress resistance genes to improve plant drought resistance. In this study, the full-length CMO gene was cloned from Hippophae rhamnoides in Qinghai, and bioinformatics analysis was carried out. Then we investigated temporal and spatial expression patterns and protein structure prediction of the CMO gene of Hippophae rhamnoides under drought stress.The subcellular localization results showed that the Hippophae rhamnoides CMO protein was located in the chloroplast; the CMO protein had no signal peptide, and its secondary structure predominantly consisted of loops. The CMO protein had many functional sites, which guaranteed the realization of protein function. In addition, the CMO protein had strong hydrophilicity and no transmembrane helix region, and the three-dimensional structure of the CMO protein was coiled with helices, strands and loops. The study of the temporal and spatial expression patterns showed that the expression of the CMO gene in Hippophae rhamnoides was markedly affected by drought stress. The expression of the CMO gene increased gradually with prolonged periods of stress and decreased until the end of stress or rehydration. There were some differences in the expression of the CMO gene in different tissues of Hippophae rhamnoides. The abundance of the expression of the CMO gene was root > leaf > stem.
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