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EFFECTS OF
PRASEODYMIUM ON FLAVONOIDS PRODUCTION AND ITS BIOCHEMICAL MECHANISM OF
SCUTELLARIA VISCIDULA HAIRY ROOTS IN
VITRO
WEI LEI1,
XIAORONG SHUI2, YUANLIN ZHOU3, SHAOHU TANG1
AND MIN SUN*1
Abstract:
In the present
study the effect of rare earth element Praseodymium (Pr) on flavonoids
production and its biosynthesis key enzymes including Peroxidase (POD;
EC 1.11.1.7), Polyphenol oxidase (PPO; EC 1.10.3.1) and Phenylanlanine
ammonialyase (PAL; EC 4.3.1.5) in hairy roots of Scutellaria
viscidula was investigated. After 7 days of suspension culture,
activity of POD, PPO and PAL and total flavonoid production showed the
same response tendency, which was ascending first and then descending
with the Pr3+ elicitor increasing, and the Pr(NO3)3
concentration reaching the knee point was 15, 25, 25, 25, 25,
ordinarily. Subsequently mechanism of flavonoids accumulation by Pr
elicitation at the biochemical level is very significant for application
of rare earth element to large scale production of plant nature drugs.
1Key
Laboratory of Eco-environments in Three Gorges Reservoir Region of
Ministry of Education, School of Life Science, Southwest University,
Chongqing 400715, China
2South
China Botanical Garden, Chinese Academy of Sciences, Guangdong 510650,
China.
3School
of Material Science and Engineering, Southwest University of Science and
Technology, Mianyang 621010, China.
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