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CHARACTERIZATION OF BEAN (PHASEOLUS VULGARIS L.) CULTIVARS GROWN IN TURKEY BY SDS-PAGE OF SEED PROTEINS

İSMET BERBER^1* AND FİKRET YAŞAR²

Abstract: In the present research, a total 28 bean cultivars grown in Turkey were characterized by the numerical analysis of seed protein profiles obtained by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique. The numerical analysis of seed protein profiles generated two major clusters at similarity levels of 82.52% or above. The first cluster included 5 reference bean cultivars at the intra-cluster similarity values of 91.25% or above. The second cluster comprised the all remaining genotypes at similarity values between 84.46% and 100%. Our results also indicated that Gina standard cultivar together with all eastern genotypes belonged to subcluster IIc, having the highest intra-cluster similarities between 95.54% and 100%. In this sense, it is suggested that probably there may be a very close genetic relationship between eastern genotypes and Gina cultivar. In conclusion, this study indicated that the numerical analysis of seed protein profiles offer no final answer to the identity of bean genotypes, however, the method can provide of extra banding pattern for the discrimination of bean genotypes cultivated in Turkey.

¹Department of Biology, Faculty of Arts and Science, Sinop University, 57000 Sinop, Turkey

²Department of Horticulture, Faculty of Agriculture, Yüzüncü Yil University, 65080 Van, Turkey

^*Corresponding author’s E-mail: ismetberber@hotmail.com