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DISCOVERY OF A NOVEL CRYSTAL PROTEIN FROM PAKISTANI BACILLUS THURINGIENSIS STRAIN TOXIC TO TRIBOLIUM CASTENEUM HERBST (COLEOPTERA:TENEBRIONIDAE)

KAUSAR MALIK AND SHEIKH RIAZUDDIN

Abstract: The biotoxicity analysis of crystal protein of some Bacillus thuringiensis strains has been carried out against the larvae of red flour beetle Tribolium casteneum Hebst. Seven isolates were found highly active against T. casteneum. The most toxic isolate SG31.11 has calculated LC₅₀ value of 0.2 ug/mg of artificial diet. Presently, the active protein of isolate SG31.11 was sequenced and data showed that it resembles with a novel Cry3 protein.

National Center of Excellence in Molecular Biology, University of the Punjab 87-West Canal Bank Road, Thokar Niaz Baig Lahore-53700, Pakistan