Back to Contents

FREE FULL TEXT PDF

HIGH FREQUENCY REGENERATION FROM SCUTELLUM DERIVED CALLI OF BASMATI RICE cv. BASMATI 385 AND SUPER BASMATI

AMNA NOOR, HAMID RASHID, ZUBEDA CHAUDHRY AND BUSHRA MIRZA*

Abstract: This study was conducted to obtain a high frequency regeneration from Basmati 385 and Super Basmati, which is a pre-requisite for transformation protocol. Seed was the explant source used in this study. Callus induction was obtained from N₆ media with 2 mg l^-1 2,4-D. Super Basmati exhibited high callus induction efficiency (93.3%) followed by Basmati 385 (90.2 %). Twenty one days old maintained calli when transferred on MS medium with different combinations of auxin-cytokinin for regeneration showed 90% frequency of plant regeneration for Super Basmati with NAA 1mg l^-1 and BAP 2.5 mg l^-1 and 83% for Basmati 385 on MS medium supplemented with NAA 1 mg l^-1 and BAP 5 mg l^-1.
 

Agricultural Biotechnology Programme, Institute of Agricultural Biotechnology & Genetic Resources, National Agricultural Research Center, Park Road, Islamabad, Pakistan.
Department of Biological Sciences, Quaid-i- Azam University, Islamabad, Pakistan.
Corresponding Author: Ph #: 9251-9255217, Fax #: 9251-9255217, abi_narc@yahoo.com