| Pak. J. Bot., 37(2): 237-242, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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MICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS IHSAN ILAHI, SHAZIA BANO, MUSARRAT JABEEN AND FAZAL RAHIM
Abstract: Callus was induced on mature caryopsis of a local variety of rice (Oryza
sativa L. cv. Swat-II). The frequency of callus induction was studied
on modified MS medium using a variety of combinations of 2,4-D and Kn.
Addition of tryptophan to different combinations of auxins and cytokinins
increased the embryogenic callus mass. Calli have been successfully
proliferated on MS supplemented with 1.0 mg/l of Kn and 0.5 mg/l of
NAA. For regeneration the embryogenic callus was cultured on MS containing
different concentrations of NAA and Kn and BAP and IAA. The somatic
embryos developed into complete plantlets on regenerative media. The
plantlets were then transferred to natural conditions for acclimatization.
Department of Botany, University of Peshawar, Peshawar, Pakistan. |
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