| Pak. J. Bot., 35(5): 829-834, 2003. | Back to Contents | ||||
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Updated: 24-11-10 | ||||
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IN VITRO MULTIPLICATION OF COFFEA ARABICA SARWAT ISMAIL, BEENA NAQVI, NAHEED ANWAR AND RABIA ZUBERI Abstract: This study was conducted to develop a micropropagation protocol for Coffea arabica, one of the most economically important plant for coffee production. Apical and axillary buds and leaf explants were cultured in modified MS medium and incubated at 28°C ± 1°C temperature in both light and dark conditions. The apical bud incubated in the medium supplemented with coconut water and L-Cysteine HCl (10 mg/L) gave the best results. Coconut water promotes growth whereas L-Cysteine HCl inhibits oxidation of phenolic compounds in the medium. Food & Marine Resources Research Center PCSIR Laboratory Complex, Karachi, Pakistan. |
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