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Pak. J. Bot., 49(1): 147-154, 2017.

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MOLECULAR CHARACTERISATION AND EXPRESSION ANALYSIS OF ACC OXIDASE GENE FROM GUZMANIA RUIZ & PAV.

LIU JIANXIN*, DING HUAQIAO, WANG WEIYONG, LIU HUICHUN AND TIAN DANQING

Flower Research and Development Center, Zhejiang Academy of Agricultural Science, 311202, Hangzhou, P.R. China

*Corresponding author’s e-mail: liujianxin2000@aliyun.com; Tel: +8657183713882

Abstract

 ACC oxidase is the last key enzyme of ethylene synthesis pathway, while ethylene is a key factor affecting flowering in ornamental bromeliad. To understand ACC oxidase gene’s characteristics and its effect on ornamental bromeliad flowering, we cloned 1504bp full-length cDNA sequence (GenBank: JX972145) and 2546bp corresponding genomic sequence (GenBank: JX972146)of GoACO1 (ACC oxidase gene) from Guzmania variety: Ostara. Prokaryotic expression study showed that expression of GoACO1 can produced a 41 KD protein precipitation in Escherichia coli DE3(BL-21); Real-time quantitative analysis showed that GoACO1 can express in all tested tissues including floral organ, bract, leaf and scape, and expression quantity in bract was the highest. Through constructing plant overexpression vector, transforming into Arabidopsis thaliana, and investigating blossom character of T2 generation seeds, we found that first flowering time of the goal Arabidopsis thaliana was 1.5 days earlier, and their peak flowering time(the number of flowering more than 50%) was 1.8 days earlier, compared with wild type one. Taken together, our results suggested that GoACO1can express in all kinds of tissues and seems to promote Arabidopsis thaliana flowering earlier.

Key words: ACC oxidase gene, Guzmania, cDNA sequence, Ostara.