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Pak. J. Bot., 47(SI): 355-358, 2015. |
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Updated: 31-12-15 | ||||
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ISOLATION AND SCREENING OF ENDOPHYTIC FUNGI FOR THE REDUCTION OF TAXOL
UMAR FAROOQ GOHAR, HAMID MUKHTAR* AND IKRAM UL HAQ
Institute of Industrial Biotechnology, GC University Lahore-54000, Pakistan *Corresponding author e-mail: hamidwaseer@yahoo.com; Tel: 042-99211634
Abstract: Taxol is naturally obtained from the inner bark of several species Taxus. But its accumulation in the Taxus is very low. Alternative way to produce taxol is through fermentation processes by using taxol producing fungi. In the present study, about 150 endophytic fungal strains were isolated from ten different indigenous plants. These strains were screened for the production of the taxol through static culture in 250ml Erlenmeyer flasks. Out of these 150 strains, about fifteen strains were found to produce the compound paclitaxel (taxol) in their broths. Among these, the strain UH-10 found to produce considerable amount of taxol in the fermentation broth. This strain was isolated from the inner bark of Taxus sp. According to characteristics of the spores and morphology of the fungal culture, strain UH-10 was identified as Cladosporium cladosporioides. The fungal taxol in the broth was detected through thin layer chromatography and further analyzed by UV spectrophotometer. Further analysis was performed with high-performance liquid chromatography (HPLC) for the quantitative estimation of the taxol. The amount of paclitaxel produced by Cladosporium cladosporioides UH-10 was 140µg/L.
Keywords: Paclitaxel, Fermentation, Static culture, Anti-cancer, High-performance liquid chromatography (HPLC).
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