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Pak. J. Bot., 47(SI): 345-349, 2015.

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OPTIMIZATION OF FERMENTATION MEDIUM FOR L-LYSINE

PRODUCTION BY CORYNEBACTERIUM GLUTAMICUM

AMJED HUSSAIN, HAMID MUKHTAR^* AND IKRAM-UL-HAQ

Institute of Industrial Biotechnology, GC University Lahore-54000, Pakistan

^*Corresponding author e-mail: hamidwaseer@yahoo.com; Tel: 042-99211634

Abstract: Corynebacterium glutamicum is an industrially important microorganism used for amino acids production. The present work describes the production of L-lysine from Corynebacterium glutamicum IIB-C9 through submerged fermentation process. Fifteen fermentation media were screened in this study for L-lysine production. Out of these fifteen media, FM:13 was found a  best one for maximum production of L-lysine i,e., 2.5g/L. The medium contained glucose, ammonium sulphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium carbonate, thiamine HCl, biotin, manganese chloride tetrahydrate and casamino acids. This medium was further supplemented with different nitrogen and carbon sources to increase the L-lysine production. Glucose, as best source of carbon and ammonium sulphate as a best nitrogen source  were optimized. Finally 3.5 g/L of L-lysine was produced after 72 hrs incubation in FM:13 medium supplemented with 7% glucose and 2.5% ammonium sulphate

Keywords: Submerged Fermentation, Culture medium, Amino acids, Optimization, Production.