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OPTIMIZATION OF INOCULUM VOLUME, FERMENTATION
MEDIUM AND AERATION RATE FOR THE PRODUCTION OF GLUCOSE OXIDASE BY
UV MUTANT STRAIN OF ASPERGILLUS NIGER AN-14
IKRAM UL HAQ*, ALI NAWAZ, HAMID MUKHTAR AND ASAD-UR-REHMAN
Abstract: In this study the effect
of inoculum size, volume of fermentation medium and aeration rate was
analyzed for UV mutated strain of Aspergillus niger AN-14 regarding
enhanced production of glucose oxidase. UV treated 10 variants showing
positive variations from parent strain were isolated and analyzed for
glucose oxidase production using shake flask fermentation. Mutant strain
AN-14 gave maximum production of glucose oxidase (44.61 U/g of cell
mass) as compared to wild strain W-47 (21.74 U/g of cell mass). About
100% increase in the production of glucose oxidase was observed by the
mutant strain. The experiments were up scaled and carried out in a 7.5 L
capacity bioreactor with working volume of 5 L. It was concluded that
65% volume of total bioreactor, 5% inoculum of the total volume of
fermentation medium and aeration rate of 0.8vvm were optimum for glucose
oxidase production by the mutant strain. The optimized yields of glucose
oxidase for wild and mutant strains were recorded as 26.48U/g of cell
mass and 50.39 U/g of cell mass, respectively.
Key Words: Inoculum, Bioreactor,
Mutant, Fermentation.
Institute of Industrial Biotechnology, GC University Lahore-54000,
Pakistan
*Corresponding author e-mail:
ikmhaq@yahoo.com; Tel: 042-99211634
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