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Updated: 18-08-15 | ||||
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RHIZOSPHERE MICROBIAL COMMUNITIES FROM RESISTANT AND SUSCEPTIBLE WATERMELON CULTIVARS SHOWED DIFFERENT RESPONSE TO FUSARIUM OXYSPORUM F. SP. NIVEUM INOCULATION
WU FENG-ZHI*1,2, CHEN SHAO-CAN1,2, CHANGCHUN-LING1,2，AN MEI-JUN1,2, ZHOU XIN-GANG1,2 AND XU WEI-HUI1,2
Abstract: Fusarium oxysporum f. sp. niveum (FON), a soil-borne pathogen of watermelon (Citrullus lanatus), can cause substantial production losses worldwide. In this study, plate culture and PCR-denaturing gradient gel electrophoresis (DGGE) methods were used to evaluate the effects of inoculation of Fusarium oxysporum f.sp. niveum on rhizosphere microbial communities of different watermelon cultivars to FON. Two methods indicated that the effects of watermelon rhizosphere microbial community of different resistance cultivars to FON were much different. Populations of culturable bacteria and actinomycetes in the rhizosphere of susceptible watermelon cultivar were significantly lower than in the resistant cultivar after inoculation (P<0.05), but the opposite result was observed for fungi. Principal component analysis of bacterial and fungal community structure also showed that the cultivar of FON-inoculated soil treatment were separated from the non-inoculated controls after inoculation, and there was clear discrimination between the susceptible cultivars and the resistant cultivars. Sequence analysis of specific bands from DGGE profiles showed that specific rhizosphere bacterial and fungal groups differed between watermelon cultivars after inoculation . Both methods demonstrated that different resistant watermelon cultivars occupied different rhizosphere microbial communities, and and disease suppression might be correlated with high microbial diversity. F. oxysporum f. sp. Niveum alters the structure and functional diversity of microbial communities associated with watermelon rhizosphere.
Key words: Fusarium oxysporum f. sp. niveum, Fusarium wilt resistance, PCR-DGGE, Rhizosphere microbial community.
1Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China 2Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in Northeast China, Harbin 150030, China *Corresponding author e-mail: wufz@neau.edu.cn |
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