Pak. J. Bot., 47(4): 1517-1520, 2015.

PROTEIN ESTIMATION AND PALYNLOGICAL STUDIES OF CANNABIS SATIVA L. POLLEN IN RELATION TO RESPIRATORY ALLERGIES

ZABTA KHAN SHINWARI^1,2*, MADIHA TANVEER¹, OSMAN YUSUF¹, ANJUM PERVEEN³AND MUNEEBA KHAN³

Abstract: Airborne pollen allergies and asthma are on a rise in the metropolitan city of Islamabad. Knowledge of allergenic pollen is limited in the area. Cannabis sativa L. or commonly known as Hemp is widely spread weed in the city. Morphological studies performed via light microscopy and SEM have shown that the pollen of Cannabis sativa are 21µm long having triporate aperture, spheroidal in shape and scaberate exine. Quantitative and qualitative analysis of pollen proteins has also be done in to recognize allergenic protein bands. Bradford’s analysis for proteins quantification has shown that the hemp pollen has 30.69 mg/g protein in fresh weight of pollen. While SDS-PAGE analysis showed 11 bands of various protein size ranging from 17kDa to 150kDa. The research findings indicate that Cannabis sativa, could be a potent allergenic pollen-producing weed that might cause serious health problems in the population of Islamabad.

Key words: Cannabis, Pollen, Allergy, Protein, Pollen morphology

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			Updated: 22-08-15
	*PROTEIN ESTIMATION AND PALYNLOGICAL STUDIES OF CANNABIS SATIVA* L. POLLEN IN RELATION TO RESPIRATORY ALLERGIES ZABTA KHAN SHINWARI^1,2, MADIHA TANVEER¹, OSMAN YUSUF¹, ANJUM PERVEEN³AND MUNEEBA KHAN³* Abstract: Airborne pollen allergies and asthma are on a rise in the metropolitan city of Islamabad. Knowledge of allergenic pollen is limited in the area. Cannabis sativa L. or commonly known as Hemp is widely spread weed in the city. Morphological studies performed via light microscopy and SEM have shown that the pollen of Cannabis sativa are 21µm long having triporate aperture, spheroidal in shape and scaberate exine. Quantitative and qualitative analysis of pollen proteins has also be done in to recognize allergenic protein bands. Bradford’s analysis for proteins quantification has shown that the hemp pollen has 30.69 mg/g protein in fresh weight of pollen. While SDS-PAGE analysis showed 11 bands of various protein size ranging from 17kDa to 150kDa. The research findings indicate that Cannabis sativa, could be a potent allergenic pollen-producing weed that might cause serious health problems in the population of Islamabad. Key words: Cannabis, Pollen, Allergy, Protein, Pollen morphology ¹Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan ²Pakistan Academy of Sciences, Islamabad, Pakistan ³Centre for Plant Conservation, University of Karachi, Karachi, Pakistan ^Corresponding author’s e-mail: shinwari2008@gmail.com*

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