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DIFFERENTIATION OF RICIN USING RAPD MARKERS

MARTIN VIVODÍK*, ŽELMÍRA BALÁŽOVÁ, ZDENKA GÁLOVÁ AND TÍMEA KUŤKA HLOZÁKOVÁ

Abstract: The aim of this study was to assess genetic diversity within the set of 111 ricin genotypes using 13 RAPD primers. For differentiation of 111 ricin genotypes 13 RAPD primers were used. Amplification of genomic DNA of 111 genotypes using RAPD analysis yielded 102 fragments, with an average of 7.85 polymorphic fragments per primer. Number of amplified fragments with RAPD primers ranged from 3 to 13, with the size of amplicons ranging from 100 to 1500 bp. The polymorphism information content (PIC) value ranged from 0.491 to 0.898 with an average of 0.764 and diversity index (DI) value ranged from 0.576 to 0.900 with an average of 0.776. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared. In dendrogram separated unique genotype RM-32 from other 110 genotypes which were further grouped into 3 subclusters (1, 2, 3). Only four genotypes were not distinguished. Using more polymorphic RAPD markers genetically close genotypes can be distinguished. Knowledge on the genetic diversity of castor can be used for future breeding programs for increased oil production to meet the ever increasing demand of castor oil for industrial uses as well as for biodiesel production.

Key words: Ricinus communis, Ricin, Genetic diversity, RAPD markers

Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Trieda A. Hlinku 2, 949 76, Nitra, Slovak Republic

*Corresponding author: vivodik.martin@gmail.com