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Updated: 18-08-15 | ||||
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STUDIES ON THE CHANGES OF PROTEIN SECONDARY STRUCTURE AND CARBOHYDRATE CONTENTS IN SEEDLING-STAGE OF ABRUS CANTONIENSIS HANCE IN DROUGHT STRESS BASED ON FTIR AND CHEMOMETRICS
FANG LAN1, DEXIN KONG3, YANQUN LI4 AND RONGSHAO HUANG2*
Abstract: To study the response mechanism of seedling-stage Abrus cantoniensis to drought stress from the level of infiltration conditions, this research uses Fourier infrared spectroscopy based on such mathematical approaches as Fourier self-deconvolution and Gaussian curve-fitting to analyze the broad characteristic absorption bands of the protein secondary structure and carbohydrates in the root, stem and leaf of seedling-stage under four drought stress treatments. The results show that the relative absorption of every type of secondary structure (namely, α-helix, β-sheet and β-turn), the α-helix/β-sheet ratio and the relative contents of starch, soluble sugars and cellulose show significant differences in the different parts of seedling-stage A. cantoniensis under different drought treatments. These differences provide a theoretical basis for investigating the response mechanisms of seedling-stage A. cantoniensis to drought stress and for advancing rational cultivation measures for this species. A new method that is fast, efficient and sensitive is presented for the study of the response mechanism of seedling-stage A. cantoniensis to drought stress.
Key words: Abrus cantoniensis, Protein secondary structure, Carbohydrates, FTIR, Chemometrics, Drought stress
1Postgraduate Department, Guangxi University, Nanning Guangxi 530004 2College of Agriculture, Guangxi University, Nanning Guangxi, 530004 3Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin, 541006, China 4Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry, South China Agricultural University, Guangzhou, 510642, China *Corresponding author’s e-mail: hrshao802@163.com; Tel: +86-3235612 |
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