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PEROXIDASE,
POLYPHENOL OXIDASE ACTIVITY, PROTEIN PROFILE AND PHENOLIC CONTENT IN
TOMATO CULTIVARS TOLERANT AND SUSCEPTIBLE TO FUSARIUM
OXSYPORUM F.SP.LYCOPERSICI
GYANENDRA KUMAR RAI1,
RAJESH KUMAR, J. SINGH, P. K. RAI2 AND S.K. RAI3
Abstract:
The total phenol
content, peroxidase and polyphenol oxidase enzyme activities and the
total protein profile in tomato cultivars (Lycopersicon esculantum
Mill.), tolerant and susceptible to Fusarium wilt disease was
studied. The tolerant cultivars of tomato viz., FEB-2, FEB-4, FloraDade
and NF-31 had significantly higher phenol content as well as peroxidase
and polyphenol oxidase activities than the susceptible ones (Sel-7,
Sel-18 and Punjab Chhuhara). The maximum peroxidase activity was
recorded in the resistant cultivar, Flora Dade (02.073unit/ml) and
minimum in the susceptible cultivar,Sel-18 (0.241unit/ml). Major
differences in soluble protein banding pattern were observed in the
susceptible and resistant cultivars. The hierarchical cluster analysis
was performed using NTSYS-pc(V.1.8) software. The dendrogram using the
average linkage between the groups, showed proximity of resistant
cultivars viz., FEB-4,FEB-2, Flora Dade and NF-31 to the wild species
with respect to similarity of banding patterns. The three susceptible
cultivars viz., Sel-7, Punjab Chhuhara and Sel-18 were grouped
separately.
Indian Institute of
Vegetable Research, Varanasi, (UP) India
1Biochemistry, Division of PBG, FOA, SKUAST-Jammu, Chatha, Jammu 18009
(J&K)
2KVK-Jammu, SKUAST-Jammu
3Division of PBG, SKUAST-Jammu, Chatha, Jammu 18009 (J&K), India
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