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Seed  protein  profiling  of  Pisum sativum L., germplasm using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) for investigation of biodiversity

Abdul Ghafoor AND MUHAMMAD ARSHAD

Abstract: Sixty-seven pea genotypes originating from 5 countries were investigated for genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on seed proteins, 25 subunits were observed and among these 20 were polymorphic. The un-weighted paired group method with arithmetic averages (UPGMA) exhibited 9 clusters which were grouped into 5 distinct clusters when plotted for first two components that contributed 62 % of variability. The genotypes from Romania and India were separated clearly that might be due to their unique genetic make up. Other genotypes were more or less scattered indicating shared protein profile that might be due to common parentage or exchange of pea germplasm by different breeders. In broader spectrum, the genotypes from various sources differed in grouping and it was difficult to establish relationship between origin and cluster pattern. The protein banding data were investigated in relation to agronomic traits evaluated for 2 years that indicated influence of polymorphic bands on quantitative traits. Particular clusters were better for specific traits that are suggested to utilize in crop improvement program.

National Agricultural Research Centre (NARC),Park Road, Islamabad, Pakistan. E-mail: ghafoor59pk@yahoo.com