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COMPARATIVE EFFICACY OF GARLIC EXTRACT AND STREPTOMYCINE SULPHATE AGAINST XANTHOMONAS CAMPESTRIS PV. CITRI (HASSE) DYE IN VITRO, AND ON THE CONTROL OF CITRUS CANKER IN GREEN HOUSE

M. ASLAM KHAN, F. IQRAM AND A. RASHID

Abstract: Streptomycine sulphate and garlic (Alium sativum) extract were tested at different concentrations against the multiplication of Xanthomonas campestris pv. citri on nutrient agar using dual culture assays. The effective concentration and their combination was then applied on the greenhouse grown citrus plants inoculated with the strain of bacterium tested in lab. The antibiotic and garlic extract inhibited the bacterial multiplication at all doses significantly (P<0.05) compared to control. Streptomycine sulphate at 1 % was most effective followed by 0.2 % and 0.1 % concentration against the multiplication X. camp. pv. citri. Garlic extract used at standard dose (S), S/5 and S/10 significantly inhibited the multiplication of X. camp. pv. citri compared to control but it was more effective when used with 0.1 % concentration of streptomycine sulphate. Thus streptomycine sulphate + Alium sativum at 0.1 % + S concentration, were the best in reducing the growth of bacterium followed by 0.1 % and S/5 and 0.1 % + S/10 combinations. Garlic extract proved effective in vitro but was not so effective on the inoculated plants grown in green house. However, its application at S/10 concentration plus 0.1 % concentration of streptomycine sulphate reduced 51.3 % canker disease over control compared to 60 % reduction in disease by the application of streptomycine sulphate alone (at 1 % concentration).

Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.