Back to Contents

FREE FULL TEXT PDF

IN-VITRO EVALUATION OF PLANT EXTRACTS AND ANTAGONISTIC FUNGI AGAINST XANTHOMONAS CAMPESTRIS PV. CITRI (HASSE) DYE

F. IQRAM, M. A. KHAN AND S. M. KHAN

Abstract: Sensitivity of Xanthomonas campestris pv. citri was tested by dual culture plate assays using 10 plant extracts viz. Acacia modesta (Phulai), Acacia nilotica (Babool), Allium sativum (Garlic), Bougainvillea spectabilis (Bougainvillea), Dalbergia sisso (Shisham), Datura alba (Dhatura), Eucalyptus cameldulensis (Sufeda), Ficus religiosa (Pipal), Olea europaea (Olive) and Phyllanthus emblic (Emblic) and six antagonistic fungi viz. Aspergillus flavus, Aspergillus niger, Baeuveria bassiana, Paecilomyces lilacinus, Trichoderma harzianum and Verticillium chlamydosporium. Except Ficus religiosa, all the plant extracts at standard concentration (75 g fresh leaves + 25 mL sterilized water) reduced the multiplication of X. camp. pv. citri significantly compared to control. Alium sativum was the best followed by A. nilotica, D. alba, D. sisso and A. modesta inducing 43, 24, 24, 23.67 and 17.78 % inhibition of the bacterial growth over control, respectively. Phyllanthus emblica, E. cameldulensis and B. spectabilis were statistically at par in their effectiveness. All the antagonistic fungi significantly reduced the bacterial multiplication than control. Aspergillus flavus was the best antagonist, while A. niger, T. harzianum, P. lilacinus and V. chlamydosporium and B. bassiana were equally effective in retarding the multiplication of X. camp. pv. citri compared to control.

Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.