14696 10.30848/PJB2027-4(7) <p> The mitochondrial genome of <em>Pinus sylvestris</em> var. <em>mongolica</em> Litv. (Pinales: Pinaceae)</p> Jian-Hua Liu, Qing Ma, Qing-Hui Ma and Sen-Yao Liu <p style="text-align: justify;"> We used Illumina and Nanopore data to complete the sequencing and assembly of mitochondrial genome of <em>Pinus sylvestris</em> var. <em>mongolica</em>, an important coniferous tree species adapted to arid and semi-arid ecosystems. The genome comprises 2,273,603 bp with a GC content of 46.80% (A: 26.56%, T: 26.65%, C: 23.47%, G: 23.33%) and features both molecule 1 and molecule 2 components. It encodes 39 protein-coding, 11 tRNA, and three rRNA genes, with notable gene duplications and intron-rich regions. Phylogenetic analysis places <em>P. sylvestris</em> var. <em>mongolica</em> within <em>Pinus</em>. Under the limited set of publicly available Pinaceae mitogenomes, <em>Pinus</em> and <em>Cathaya</em> are adjacent in the tree</p> <p> &nbsp;</p> inpress 2027 59 4 1 9