13860
10.30848/PJB2026-12(17)
<p>
Breeding, growth characteristics and fungal inhibitory effect of thermotolerant <em>Bacillus velezensis</em> derived from distiller’s grains</p>
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Yazi Li, Aamir Rasool, Jinxiao Yang, Zhixin Zhang, Siyuan Xing, Ziyang Wang, Ziyue Zhang, Xiqing Zhang, Yongshan Fan and Ke Xu
<p>
This study used a starter strain, <em>Bacillus velezensis</em> TS01, isolated from distiller’s grains. To directionally breed thermotolerant strains at 42-46°C for 532 h and 50 generations, EVOL (cell adaptive evolution technology) was used. Strain 46-1 has a growth of 250% higher than that of the original strain at 46°C, with stable entry into the logarithmic phase and a prolonged stationary phase. It grew optimally at 2% ethanol and 4% NaCl, grew well at pH 3, and produced amylase, protease, and cellulase. When it was tested against six pathogenic fungi, it exhibited the highest inhibition rate against <em>Eutypella microtheca </em>(72.60±0.99%). Then, it was followed by 58.60±4.24% and 56.53±0.11% against <em>Mortierella gemmifera</em> and <em>Talaromyces fuscoviridis</em>, respectively. 39.49±0.61% against <em>Mortierella elongata</em>; 38.78±0.44% against <em>Fusarium oxysporum </em>and 20.68±1.45% against <em>Trichoderma harzianum</em>. This research study provides a basis for greater biocontrol efficacy of <em>B. velezensis</em> in a high-temperature environment and for developing microbial fungicides</p>
inpress
2026
58
12
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