11911
10.30848/PJB2024-6(32)
<p>
EST-SSR marker development based on de novo transcriptomic assembly of <em>Aesculus chinensis</em> Bunge</p>
Yu Ling Li, Xin Mao, Tian Xu Sun, Cui Lan Liu, Yuan Shuai Zhang, Yuan Fu Dong and Xiu Hong Mao
<p style="margin-left:.25in;">
<em>Aesculus chinensis</em> Bunge, a deciduous tree in the genus <em>Aesculus</em> of the Hippocastanaceae family, has ornamental, economic, and medicinal values. But the scarcity of comprehensive genetic data hinders the utilization of this species, especially the EST-SSR markers in <em>A. chinensis </em>Bunge have not been reported previously. In this study, the transcriptomes of<em> A. chinensis </em>Bunge were assembled from novo, which enabled the identification of 33,365 unigenes with a mean length of 1348 bp. The sum of 25450 (76.27%) unigenes showed similarities with at least one database including Nr (71.39%), Swiss Prot (57.03%), GO (53.31%), KO (27.13%) and KOG (19.27%). Based on the transcriptome data collected for <em>A. chinensis</em> Bunge, 16,103 SSR loci were detected with a distribution density of one SSR per 2.79 kb. The most dinucleotide and trinucleotide repeats were AG/CT and AAG/CTT. Then 58 primer pairs were randomly selected for polymorphism validation and 31 primer pairs were polymorphic among eight <em>Aesculus</em> samples. There were 167 alleles detected, varying from 2 to 9 with a mean of 5.387 alleles per locus. According to the allelic variation, 7 primers were capable of distinguishing the 8 <em>Aesculus</em> samples from 6 species. These findings will be useful for the conservation, genetic diversity assessment and molecular identification of <em>Aesculus</em> resources.</p>
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Published
2024
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6
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