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PJB-2006-9

UPTAKE OF NITRATE AND AMMONIUM ION BY CELL SUSPENSION CULTURES OF VIGNA RADIATA

SAADIA AFZAL, MUHAMMAD ZIA AND *M. FAYYAZ CHAUDHARY

Abstract

A study was conducted to examine the effect of pH on the uptake of NH4+ and NO3- by the cell suspension culture of Vigna radiata. Uptake of NH4+ was faster than NO3- under all conditions of the medium whereas uptake of NH4+ and NO3- both were affected by the pH of the medium. Maximum uptake of NH4+ was observed on pH 4.5. Uptake of NO3- was faster in the culture having pH 7.0. It is therefore concluded that pH of the medium influence the uptake of both NH4+ and NO3- in the cell suspension culture of Vigna radiata.

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