PJB-2005-80
HIGH FREQUENCY REGENERATION FROM SCUTELLUM DERIVED CALLI OF BASMATI RICE cv. BASMATI 385 AND SUPER BASMATI
AMNA NOOR, HAMID RASHID, ZUBEDA CHAUDHRY AND BUSHRA MIRZA*
Abstract
This study was conducted to obtain a high frequency regeneration from Basmati 385 and Super Basmati, which is a pre-requisite for transformation protocol. Seed was the explant source used in this study. Callus induction was obtained from N6 media with 2 mg l-1 2,4-D. Super Basmati exhibited high callus induction efficiency (93.3%) followed by Basmati 385 (90.2 %). Twenty one days old maintained calli when transferred on MS medium with different combinations of auxin-cytokinin for regeneration showed 90% frequency of plant regeneration for Super Basmati with NAA 1mg l-1 and BAP 2.5 mg l-1 and 83% for Basmati 385 on MS medium supplemented with NAA 1 mg l-1 and BAP 5 mg l-1.
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