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Ectopic expression of Cu/Zn-SOD gene from Ceriops tagal enhances salinity tolerance of yeast
Abstract
Ceriops tagal is a typical non-salt secretion mangrove plant while it has a strong salt resistance. However, the mechanism of C. tagal underlying salt stress remains largely unknown. Superoxide dismutase (SODs) is the first line of defense system against active oxygen in plants. In order to investigate whether SOD involves in salt resistance in C. tagal, an antioxidant enzyme related gene (Cu/Zn-SOD) was isolated from the root of C. tagal and expressed in yeast in this study. The transgenic yeast INVSc1 could effectively express the Cu/Zn-SOD protein and improve the salt tolerance of yeast significantly. This is the first study about cloning a Cu/Zn-SOD gene from the root of C. tagal and revealing its role in salt stress resistance in yeast.
