Paper Details

PJB-2005-52

RFLP-BASED RELATIONSHIP OF PAKISTANI ISOLATE OF BANANA BUNCHY TOP VIRUS WITH SOUTH PACIFIC VIRUS GROUP

TAHIRA YASMIN, S.M. SAQLAIN NAQVI*, HUSSAIN SHAH AND SAIF KHALID
Abstract

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Restriction Fragment Length Polymorphism (RFLP) technique was employed to investigate the relationship of Pakistani isolates of banana bunchy top virus (BBTV) with Asia and South Pacific strains. The PCR amplified product of BBTV DNA component I was digested with nine different restriction endonucleases (Bam HI, EcoRI, EcoRV, HaeIII, HincII, HindIII, PstI, RsaI and SmaI) and analyzed by agarose gel electrophoresis. HaeIII resulted in three DNA fragments of 505

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