PJB-2005-32
MICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS
IHSAN ILAHI, SHAZIA BANO, MUSARRAT JABEEN AND FAZAL RAHIM
Abstract
Callus was induced on mature caryopsis of a local variety of rice (Oryza sativa L. cv. Swat-II). The frequency of callus induction was studied on modified MS medium using a variety of combinations of 2,4-D and Kn. Addition of tryptophan to different combinations of auxins and cytokinins increased the embryogenic callus mass. Calli have been successfully proliferated on MS supplemented with 1.0 mg/l of Kn and 0.5 mg/l of NAA. For regeneration the embryogenic callus was cultured on MS containing different concentrations of NAA and Kn and BAP and IAA. The somatic embryos developed into complete plantlets on regenerative media. The plantlets were then transferred to natural conditions for acclimatization.
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