PJB-2019-246
Genetic diversity and relationship of Dongting biluochun tea germplasm in Suzhou revealed by SSR markers.
Qiongqiong Zhou, Huan Li, Tran Xuan Hoang, Xu Ruan, Yue Zhang, Emmanuel Arkorful, Xuan Chen, Kang Sun and Xinghui Li
Abstract
Dongting Biluochun (DTBLC) tea is one of the top famous tea in China, which is originated from Suzhou city, Jiangsu Province, China. Generally, DTBLC tea is processed using ‘Dongting population’, which have a great genetic diversity. However, little information has been found so far on its genetic diversity. In order to unveil the relationship among the DTBLC germplasm, 68 tea accessions collected from Suzhou geographical origin growing area were analyzed by using 36 pairs of polymorphic simple sequence repeat (SSR) primers. Finally, a total of 68 tea accessions generated 158 alleles with a mean of 4.39 alleles per locus and 314 genotypes with a mean of 8.72. The polymorphism information content (PIC) was between 0.09 and 0.76, and the mean value was 0.53; the gene diversity index (H) ranged from 0.10 to 0.79, with an average of 0.56, while the average observed heterozygosity (Ho) was 0.50 varied from 0.04 to 0.71. The coefficient of the genetic similarity of SSR makers among the tea accessions ranged from 0.49 to 0.89. These results indicated that a considerable genetic diversity existed among these tea accessions. According to the clustering results, 68 tea accessions were clustered into 5 categories with no obvious geographical distribution characteristics based on the unweighted pair group method of arithmetic mean (UPGMA), which hinted that the territorial division of protection for geographic indication-DTBLC tea was appropriate. However, dendrogram based on Nei's genetic distance revealed complex genetic relationships among DTBLC tea germplasm resources, and these collections possessed impressive level of genetic diversity which could be important for subsequent selective breeding, utilization, and conservation of tea germplasm resources in Jiangsu, China.
To Cite this article:
Zhou, Q., H. Li, T.X. Hoang, X. Ruan, Y. Zhang, E. Arkorful, X. Chen, K. Sun and X. Li. 2019. Genetic diversity and relationship of Dongting biluochun tea germplasm in Suzhou revealed by SSR markers. Pak. J. Bot., 51(3): DOI: http://dx.doi.org/10.30848/PJB2019-3(31)
Download
