PJB-2018-407
Symptoms and reduction of hyperhydricity associated with the shoots regenerated from callus Salvia santolinifolia.
Tour Jan
Abstract
Summary
Hyperhydricity is a frequent problem in the regenerated shoots of Salvia santolinifolia from callus. The callus formed at the base of In vitro regenerated shoots on MS medium with BA (4.0 mg/l) were utilized for shoots regeneration. This callus formed numerous hyperhydric shoots on BA (4.0 mg/l) supplemented media. The problem of hyperhydricity was condensed to 37.82% by evaluating strength of medium, cytokinins types & concentrations and containers size. We consider this disorder as the results of high humidity in the culture containers which encourage the absorption of water by the cell. We discuss that hyperhydric symptoms (structures) like glassy appearance and wrinkled leaves resulting from waterlogging of the cells. Our experimental data exposed that BA at the level of 3.0 mg/l in full MS medium by using large size containers of 300 ml was suitable for normal shoots induction and development from callus of this species.
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