PJB-2018-393
Enhancement of the Three Effective Constituents in Notopterygium incisum by
Yehong Guo
Abstract
Qianghuo, the subterraneous root of Notopterygium incisum, has been used as an antirheumatic and analgesic medicine in China. Isoimperatorin, notopterol, and ferulic acid are the main bioactive components described in Pharmacopoeia. In this study, a reverse phase high-performance liquid chromatography (RP-HPLC-DAD) method based on that in the “Chinese Pharmacopoeia” was established for the determination of these three bioactive constituents. The different altitude areas of Qianghuo were determined and the influence of cultivation on the quality of Qianghuo was also analyzed. The results showed that contents of the three constituents of Qianghuo cultivated in Lanzhou(lower-altitude) meet the criteria of the Pharmacopoeia of the People's Republic of China; however, the constituents of Qianghuo cultivated in Tianzhu do not meet the standard of the Chinese Pharmacopoeia. The contents of the three constituents in the stems and leaves of Notopterygium incisum were also analyzed. The results indicated that the contents of the three components in stems and leaves do not accord with the standards set in the Pharmacopoeia. The RP-HPLC method for the determination of the main bioactive component was sufficiently reliable and accurate. Furthermore, this method could be used in the evaluation of Qianghuo quality. This study has indicated that the altitude and farmland management could affect not only the growth but also the contents of the main constituents in Qianghuo.
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