PJB-2018-364
MORPHOTYPING AND MOLECULAR ANALYSES OF ECTOMYCORRHIZAL FUNGAL OTUS ASSOCIATED WITH PINES IN HIMALAYAN REGION OF PAKISTAN
SAMINA SARWAR
Abstract
Pinaceae is an ectomycorrhizal plant family including many economically important conifers i.e. Pinus wallichiana, Abies pindrow, Pinus roxburghii, etc. which form symbiotic association with mushrooms in the form of ectomycorrhizae that help by enhancing tree growth, nutrient cycling, nutrient uptake, etc. During investigation on diversity of belowground mycoflora from moist temperate coniferous forests of Himalayan range in Pakistan, ectomycorrhizae belonging to fungal genera Inocybe, Rusulla and Trichophaea were found associated with roots of Pinus wallichiana and Abies pindrow. These ectomycorrhizae were described morpho–anatomically by observing root morphology, mantle structure, specialized hyphae and rhizomorph arrangement, and phylogenetically by amplifying ITS region of rDNA. Emphasis was on molecular–phylogenetic analysis of the ITS region for identification. Phylogenetic analysis place these ectomycorrhizae with their respective genus retrieved from GenBank. These ectomycorrhizae seems new to science world hence specific names will be given after identifying their sporocarps. After BLAST and comparison with related taxa, sequences were submitted in the GenBank.
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