PJB-2018-321
BIOLOGICAL ACTIVITIES OF WITHANIA SOMNIFERA (L.) DUNAL.USED IN TRADITIONAL MEDICINE IN PAKISTAN
Gul Jan
Abstract
ABSTRACT The present study was focused to evaluate the phytochemical, antimicrobial and cytotoxic activities of ethanolic extract of Withania somnifera (L) Dunal. The extraction of ethanol in W. somnifera leaves has flavonoids, alkaloids, saponins and tannins. These activities were confirmed by in vitro cytotoxic studies in which W. somnifera showed the maximum control over HeLa cell lines with the IC50 value of 40 μg/ml. Ethanolic extracts were used against infectious pathogenic bacteria i.e Bacillus atropeous, Bacillus subtlilis, Staphylococcus aereus (Gram positive bacteria) and Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and Klebsela pneumonia (Gram negative bacteria). In vitro antimicrobial activity disc diffusion method was used. The activity was expressed as mg/µl. Ethanolic extracts of W. somnifera was found active against all microbes. With phytochemical activities, the toxicity, pharmacological activities and establishing safety was required for the further studies. This research supports the uses of the selected medicinal plant against bacterial infection. Key words: Phytochemical; In vitro Antimicrobial activity and cytotoxicity; Withania somnifera
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