PJB-2018-264
Genetic Diversity of Mountain Garlic (Allium ochotense Prokh. & Allium microdictyon Prokh.) Based on the nrDNA ITS Region Sequence
SHI-LIN ZHENG
Abstract
Mountain garlic (Allium ochotense Prokh. & Allium microdictyon Prokh.) is an edible perennial herb and has been used as a vegetable or as a traditional medicine in South Korea, Japan, and China. In this study, 25 samples from eight regions of South Korea were collected to analyze genetic diversity and relationships based on the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region. The total ITS region was successfully amplified using the universal primer site, ITS4/ITS5, and the GenBank accession numbers, MF599168- MF599192, were botained. The sequence length of the total ITS region of 25 samples is approximately 695 bp. The length of ITS 1 ranged from 262 bp to 270 bp, and the length of ITS 2 ranged from 253 bp to 267 bp. According to the results of sequence alignment, two groups (Group I and Group II) were clearly divided into different species rather than geographical regions. Nearer geographical position, similar climates, and growing environments might be the main reasons for this grouping. Thus, to further research the genetic diversity among mountain garlic from different regions, more varieties and wider geographical locations should be investigated in the future.
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