PJB-2018-240
FIRST REPORT ON THE GENETIC EVALUATION OF ALLIUM CEPA L. (ONION) LANDRACES GROWING IN MALAKAND DIVISION, KHYBER PAKHTUN KHWA, PAKISTAN
KHALID KHAN
Abstract
Exploration and genetic evaluation of local landraces is beneficial to the modern agriculture, which broaden the gene-pool for the plant breeders and enhance crop performance. In this research work we investigate the genetic variability of 60 local Allium cepa (L.) landraces collected from Malakand division through Agro-morphological and biochemical markers. The local landraces of Allium gene-pool having a rich-allelic variation. After exploration the field evaluation has been exhibited high phenotypic variability in both qualitative and quantitate traits. Two sets of experimental field were designed for Agro-Morphological parameters; one is planted in District Swat while the second at Botanical garden University of Malakand District Dir lower. All the parameters noted as per descriptor IPGRI 1994. The fungal disease downy mildew was noted in 16 landraces (26.67 %), white patches 15 (25 %), purple blotch 25 (41.67%) while 35 landraces (58.33 %) examined with no disease. The disease damping off was scored in 1.67% samples at seedling stage out of 60 investigated landraces of Onion. Among the quantitative trait maximum level of GD was scored for pseudo-stem height (22.00%) and 10-bulb weight (21.73%). Correlation analysis revealed that stem height is highly significantly positively correlated with stem, bulb and plant height (r = 0.929 & r = 0.432) while leaf length was highly significantly and positively correlated with total plant height (r = 0.944) and stem plus bulb showing strong positive correlation with total plant height (r = 0.502). Seed storage proteins were detected through Sodium Dodecyl Sulphate, Polyacrylamide Gel Electrophoresis (SDS-PAGE). The high level of variation was noted in B1 (0.95 %) followed by B4 (0.93 %), B2 (0.92 %), B3 (0.88 %), B5 (0.78 %) and B8 (0.53 %) polymorphism respectively. Similarly, B14 (0.13%) revealed low level of i.e. B10, B7 (.17%), B6 (.22%), B11 (.25%), B13 (.30%) and B9 (.45%). Similarly, results revealed that low level of variation was found in B10 (.17 %), B7 (.17 %), B6 (.22 %), B9 (.45 %), B11 (.25 %), B13 (.30 %) and B14 (0.13 %). The research aim was to choose the promising varieties based on their yield production and resistant to diseases. Key words: Allium cepa, Genetic diversity, Agro-Morphology, SDS-PAGE, Dendogram. Statistical analysis
To Cite this article:
Download
