Paper Details

PJB-2018-235

Transcriptome reveal the diversity of polyketide synthase genes from the lichen-forming fungus Ramalina conduplicans

Yi Wang
Abstract

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Ramalina conduplicans is an edible lichen in China, which produce many bioactive polyketides. To reveal their polyketides biosynthetic genes, the transcriptome of lichen forming fungus R. conduplicans producing antibacterial compound was sequenced. Six different type polyketide synthases (RcPKS1; RcPKS2; RcPKS3; RcPKS4; RcPKS5; RcPKS6) from R. conduplicans were cloned by fusion PCR. Phylogenetic analysis of ketoacyl synthase domains showed that RcPKS1 and RcPKS2 belonged into Reducing polyketide synthase (PKS); RcPKS3 belonged into non-reducing PKSs (clade V) which synthesize single aromatic ring polyketide, such as orsellinic acid; RcPKS4 belonged into non-reducing PKSs (clade I) which synthesize pentaketide polyketide; RcPKS5 and Rc PKS6 belonged into non-reducing PKSs with MeT domain (clade III) which include the PKSs involved in methyl-orcinol polyketide. RT-PCR analysis results showed that SY (soya peptone and yeast extract) stimulated all of six PKS genes expression.

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