PJB-2018-212
Response of date palm pollen tube growth to different storage conditions and periods
Maryam
Abstract
Date palm (Phoenix dactylifera L.) is the fifth major fruit crop after citrus and mango in Pakistan. Date palm pollen remains viable for a single growing season. Air dried pollen was enclosed in sealed capsules and stored at 4°C, -20°C and -80°C. The growth rate of pollen tube of date palm pollen grains was measured after 1,3,6 and 12 months of storage stored either in refrigerator or freezer at 4oC, -20oC and -80oC. Pollen tube length was observed under the microscope at 200X magnification using micrometer. The growth of pollen tube was found to be function of storage conditions and storage periods. The pollen tube elongation rate of pollen grains stored at 4oC and -80oC decreased during storage while pollen grains stored at -20oC maintained high rate of pollen tube growth when incubated at 30oC over storage period of 12 months.
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