PJB-2018-162
ANTIMICROBIAL ACTIVITY OF NOVEL METABOLIC EXTRACTS OF MUTANT VARIANTS OF XANTHORIA PARIETINA
Sikander Ali
Abstract
A biological active secondary metabolite parietin was extracted from wild-type and mutant strain of lichen Xanthoria parietina. Six different solvents (acetone, ethanol, methanol, hexane, chloroform and distilled water) were used to extract parietin. The parietin activity was found to be maximal when extraction was carried out with 3 ml of methanol at 30ºC for 1h. All the extracts were further proceeded to carry out the antimicrobial activity against different strains of bacteria and fungi. Methanol extracts showed strong antibacterial activity followed by ethanol and hexane extracts. After optimizing the conditions, the parietin was extracted from ethyl methane sulphonate (EMS) and methyl methane sulphonate (MMS) treated strains of X. parietina. The mutant concentration of 0.5 mM exhibited strong parietin activity as compared to other concentrations. The extracts of EMS treated mutant variant revealed strong antimicrobial activity (22 mm for bacteria & 19 mm for fungi) as compared to the wild-type and MMS treated strain. It was concluded that by increasing mutant exposure time, the parietin activity decreased in terms of its antimicrobial activity.
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