Paper Details

PJB-2018-149

MYCOFLORA ASSOCIATED WITH PHASEOLUS VULGARIS L. SEEDS IN AZAD JAMMU & KASHMIR AND THEIR IMPACT ON SEED GERMINATION

SHOUKAT HUSSAIN
Abstract


Seed of Phaseolus vulgaris L. collected from different localities of State of Azad Jammu and Kashmir were evaluated for seed associated Mycoflora by standard blotter paper method and Agar plate method. Among various factors responsible for low yield and high mortality rate of seedlings before and after emergence, One is seed associated Mycoflora. The seed samples were observed highly contaminated with fungi belonging to eight genera i.e. Alternaria, Aspergillus, Curvularia, Drechslera, Fusarium, Mucor, Penicillium and Rhizopus.The highest infection percentage was recorded from Trarkhal, Bagh and Chakaar samples, i.e. 24.75A, 18.25B and 13.25C respectively. Least infection percentage was observed on Lawaat (3.00H) and Kail (11.8E) samples. Impact of fungal presence on seed germination was elucidated by standard rolled paper towel method. Lawaat and Kail samples show better germination 94% and 92% respectively. Incidence of fungi was recorded high and low germination in Trarkhal and Palangi samples 43% and 22% respectively. Use of different antifungal treatments found effective in increasing germination percentage and health of seedlings. Efficacy of management was evaluated by using CRD2 Factorial test. The interaction between treatments and localities was significantly different at 0.05 level of significance. Benomyl was more effective in increasing seed germination with 76.37 mean. Locality of Lawaat shows the highest germination percentage with different treatments having 96.00 mean value.The best interaction was observed between treatment 2 and locality 2, i.e. 98.00 while least interaction was calculated between treatment 1 and locality 6 i.e. 15.00.

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