PJB-2018-109
GENETIC DIVERSITY OF CORYPHA SPP. IN THAILAND BASED ON AFLP ANALYSIS
PONGTHEP SUWANWAREE
Abstract
In the past, the Corypha plants were found nationwide in Thailand. Currently, Corypha remains rare in natural habitats because people have turned forests into agricultural and urban areas, they also cut the shoots and trunks for economic benefits as a result, Corypha plants were destroyed. Nowadays, only Thaplan National Park has an extensive natural population of C. lecomtei. Therefore, to study the genetic diversity and variation among Corypha species, AFLP technique was used in the present study. Leaf samples of Lan Pha (C. lecomtei Becc.), Lan Wat (C. umbraculifera L.) and Lan Pru (C. utan Lam.) were collected from different localities in Lampang, Khon Kaen, Prachin Buri, Nakhon Pathom, Surat Thani and Satun Provinces. Four AFLP primers (EcoRl+CAA/Msel+GAA, CAA/GAG, CAG/GAT and CAG/GCA) were employed for genetic diversity and variation of 65 genotypes of Corphya spp. A total of 217 AFLP fragments were detected. AFLP data analysis was done by using NTSYS software version 2.2. All genotypes were grouped to seven clusters in the dendrogram. The average observed heterozygosity of C. lecomtei, C. umbraculifera and C. utan was between 0.165–0.209, 0.220 and 0.072–0.086, respectively. The observed percent polymorphic loci of C. lecomtei, C. umbraculifera and C. utan was between 49.30–59.44%, 76.03% and 19.81–24.42%, respectively. The results indicated that genetic variations of Thai Corypha populations decreased, leading to a high risk of extinction. Key words: Corypha, Genetic Diversity, AFLP analysis, Polymorphism.
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