PJB-2018-74
EFFECTS OF DISINFECTION CONDITIONS AND CULTURE MEDIA ON IN VITRO GERMINATION OF SEA DAFFODIL (Pancratium maritimum)
Sara Yasemin
Abstract
In this study, we investigated the disinfection methods and germination conditions for seeds of sea daffodil (Pancratium maritimum). Seeds were exposed to 20% and 40% NaOCl (Sodium hypochlorite) solutions for 15, 20, and 25 minutes, as disinfection treatments. Germinated seed number and percentage of seed germination and plant formation was evaluated to detect the best disinfection treatment. The rate of 20% NaOCl was the most appropriate concentration in terms of percentage of seed germination (58%) and plant formation (34%). Effects of three culture media on seed germination, plant formation, and growth were investigated: A. Agar (4 gl-1) with sucrose (30 gl-1); B. MS (Murashige - Skoog) with agar (4 gl-1) and sucrose (30 gl-1); C. MS with agar (4 gl-1), sucrose (30 gl-1), and plant growth regulators (1 mgl-1 BA, 0.1 mgl-1 2,4-D). Evaluated plant growth parameters were total plant weight; number, width, and length of leaf; thickness and length of root; and diameter, length, and weight of bulb. At the end of the study, all culture media were found to be successful in terms of in vitro germination of the seeds. The most suitable medium for plant formation was found to be Medium B (52%) followed by media A (25%) and C (18%). When all the plant growth parameters were considered, Medium B was the most appropriate culture medium. This is an innovative report in that it has been thoroughly investigated in terms of disinfection practices and seed germination media with different evaluation parameters.
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