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Paper Details

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PJB-1993-34

DETECTION OF SEED-BORNE MYCOFLORA IN PINUS GERARDIANA

ZAKIA BILGRAMI AND ABDUL GHAFFAR

Abstract

Using agar plate, blotter and deep freezing method, 14 genera and 26 species of fungi were isolated from fourteen seed samples of Pinus gerardiana. About 70% of the samples were infected with Aspergillus flavus and A.niger with an infection range of 4 to 56% and 4 to 100% respectively. Agar plate method yielded greater number of fungi (19) as compared to deep freezing method (13) and blotter method (12). Deep freezing method was found superior to standard blotter and agar plate method for the detection of Altemaria, Cladosporium and Fusarium spp.

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