PJB-2017-890
ANALYSIS OF POPULATION VARIATION AND GENETIC COMPARISON OF THE MAIZE GENOTYPES WITH SIMPLE SEQUENCE REPEAT MARKERS
Madeeha Taimur
Abstract
Understanding genetic diversity among and within inbred lines plays an important role in maize improvement. Simple sequence repeats (SSR) are co-dominant markers used to find the genetic diversity and accelerate the selection process. The aim of the current study was to genetic diversity among 5 maize populations with a total of 4 SSR. The 4 SSR markers amplified a total of 6 alleles Average gene diversity (GD) detected was 0.419 for 4 SSR loci among maize genotypes. Maximum GD in the maize population was observed at SRR loci umc2089 (0.686) while minimum was detected for primer umc2337 (0.2557). The PIC values ranged from 0.87 (umc1997) to 0.90 (umc1899) with an average of 0.87. The proportion of genetic diversity between the populations according to Nei’s F-statistics (GST) ranged between 0.009 (umc1899) and 0.566 (umc2337), average being 0.164. Dendogram was constructed with 4 SSR markers using Mega 6 software, 15 maize genotypes were clustered in the 2 main clusters which showed genetic polymorphism in maize populations. The present study identifies the diversity and genetic distance between maize genotypes and the information can be used to select parental lines for breeding programmes.
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