PJB-2017-841
Accumulation of Nectria haematococca in soil, a clue for the replant disease of Panax notoginseng revealed by 454 pyrosequencing
Ling Yang
Abstract
Panax notoginseng (Burk.) F. H. Chen (Sanchi or Sanqi in Chinese) is a well-known and valuable traditional Chinese herbal medicine. It is currently planted on > 20,000 hm2 with an output value of more than 10 billion RMB per year. However, the replant disease characterized by severe root rot has become a serious problem for the P. notoginseng industry, and the etiology of replant disease in P. notoginseng remains ill-defined. The microbial communities in the rhizosphere and bulk soils of P. notoginseng that had been planted 1, 2 and 3 years previously were investigated using deep amplicon pyrosequencing and compared by principal component analysis (PCA). The microbial communities in the rhizosphere and bulk soils were represented by 94,253, 18,871 and 15,116 reads that were obtained from archaeal, bacterial and fungal PCR amplicons, respectively. A principal component analysis showed that rhizosphere and bulk soils shaped similar archaeal and bacterial communities. However, fungal communities were greatly different between bulk and rhizosphere soils, and the fungi in the rhizosphere soils had a high diversity level. Nectria haematococca (asexual name Fusarium solani), the cause of root rot, was found to accumulate as a consequence of the continuous cultivation of P. notoginseng. The accumulation and long-time survival of N. haematococca may be an important clue in the study of the mechanism of replant disease of P. notoginseng, which is characterized by severe root rot.
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