PJB-2017-725
In vitro propagation of Globba siamensis Hemsl. (Zingiberaceae), a high potential ornamental plant from Thailand
Rodjanacorn Chuengpanya
Abstract
Globba is a candidate for use as an economic ornamental plant. This plant has gorgeous inflorescence that attracts great interest from observers. Moreover, the demand of Globba from the floral industry is increasing every year. Thus, Globba siamensis Hemsl. was selected of use as a model new economic ornamental plant in this study. To produce large quantities of plantlets to supply the floral industry in the near future, an efficient protocol for in vitro mass propagation of this plant was established. The leafy-shoot base was found to be the most suitable explant for micropropagation. Leafy-shoot bases at a height of 1.5 cm were excised from in vitro vigorous plantlets that had a height of 5-6 cm and were cultured on a Murashige and Skoog (MS) medium that was composed of 3% sucrose, 7% agar and various concentrations of N6-benzyladenine (BA; 0-35.52 µM) for 8 weeks. Then, the regenerated plantlets were transferred to a BA-free MS agar medium for another 4 weeks. After a total of 12 weeks of culture, G. siamensis that had been cultured on a MS agar medium supplemented with 2.22 µM BA, produced the highest number of new leafy-shoots (40.60 leafy-shoots/explant), with an average height of 19.48 cm. The highest number of roots (287.40 roots/explant) with an average length of 4.52 cm was also found with this medium. Plantlets raised in vitro were acclimatized under a saran house and showed an 80% survival rate. This protocol could be applied to the large-scale proliferation of this valuable plant.
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