PJB-2017-683
PHYTOCHEMICAL SCREENING, METAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS RECIPES USED AGAINST DIARRHEA
Dr. Ruqia Nazir
Abstract
Abstract The present study explore the phytochemical composition, heavy metal analysis and antibacterial activity of six medicinal plants i.e. Terminalia chebula Retz, (fruits), Aegle marmelos L., (fruits) Curcuma longa L., (rhizomes), Syzygium aromaticum L., (flower buds), Piper nigrum L. (seeds), Cinnamomum cassia L. (barks) and its two remedial recipes used against diarrhea by local herbal practioner (Hakeems). A preliminary phytochemical screening of various extracts like methanol, chloroform, n-hexane and distilled water revealed the presence of alkaloids, flavonoids tannins and saponins by using standard procedures. The quantative phytochemical screening show that alkaloids (27.84 %) flavonoid (61.21%) and saponins (6.32%) were in higher quantinty in Terminalia chebula. The metal analysis for Cd, Ni, Pb, Fe, Cr, Cu and Zn was carried out by atomic absorption spectrometer. The analysis showed that Cr, Fe and Pb are present in very high concentration in medicinal plants and in their recipes. The antibacterial activities of the crude methanol, chloroform, n-hexane and distilled water extract was performed by using agar well disc diffusion assay against four bacterial strains namely E-coli, salmonella, shigella and MRSA. Maximum zones of inhibition in methanolic, aqueous, chloroform and n. hexane extract were seen in recipe 2 against shigella (22.16 ± 0.47 mm), recipe 2 against shigella (20.33 ± 0.24 mm), Recipe 1 against shigella (20.3 ± 0.29 mm) and recipe 2 against E-coli (30.23 ± 0.12 mm) respectively. Furthermore the recipe extracts are more active against the tested bacterial strains then the extracts of the individual plant. Therefore the use of herbal plants and their recipes as a source of drugs in traditional medicinal system for curing various diseases are justified.
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