PJB-2017-667
Isolation, evaluation and characterization of a constitutive plant promoter from potato Aquaporin gene
Hira Mubeen
Abstract
Background: Intrinsic protein (PIP1) belongs to aquaporin gene family and is one of the abundantly expressed genes in plants. In this study, the promoter of aquaporin PIP1 gene was identified, analyzed and retrieved from high throughput genomic sequence (HTGS) database. The cis-acting regulatory elements and transcription start sites of promoter were identified through different bioinformatics tools. Results: The aquaporin promoter was isolated from Solanum tuberosum and was initially cloned in TA vector (pTZ57R/T) and later transferred to plant expression binary vectors, pGR1 and pGA482 for transient and stable expression analysis. The transient and stable GUS expression assays for this promoter in various tissues of Nicotiana tabacum indicated its functional importance in regulating gene expression in a constitutive manner. Conclusion It was concluded that the aquaporin promoter is constitutively expressed with a strength equivalent to CaMV 2X35S promoter. The promoter isolated through these studies may be effectively substituted in plant genetic engineering for the crop improvement.
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