PJB-2017-207
ROLE OF OsWAK124, A RICE WALL-ASSOCIATED KINASE, IN RESPONSE TO ENVIRONMENTAL HEAVY METAL STRESSES
XIAOYAN YIN AND XUEWEN HOU
Abstract
Members of the Arabidopsis cell wall-associated kinase (WAK) family play important roles in both development and stress responses. There are about one hundred and twenty fiveOsWAKs annotated in the rice genome but their functions in rice growth and development are largely unknown. In this paper, we reported a functional role of the OsWAK124 (Os12g0266200) in rice heavy metal responses. Confocal GFP experiments located OsWAK124 in the cell wall and analyses of OsWAK124 promoter⸬GUS transgenic lines suggested that OsWAK124 promoter is primarily active at the meristematic tissues under normal growth condition. Under stress conditions, however, OsWAK124 promoter activity is induced in non-meristematic tissues, such as leaf, stem and root, and the activity in the meristematic tissues is further enhanced. Various transgenic rice lines carrying either RNA interference (RNAi) or overexpression constructs were generated. Transgenic lines were tested for their responses to various stress conditions including salicylic acid, NaCl, AlCl3, CuSO4 and CdSO4. Our analyses showed that rice seedlings overexpressing OsWAK124 are more resistant to the three tested heavy metals (Al, Cu, and Cd), which suggested that OsWAK124, like some Arabidopsis WAK members, plays a role in environmental heavy metal stress responses.
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