Paper Details

PJB-1989-40

Separation of tomato yellow top virus and beet western yellows virus from doubly infected plants.

Sher Hassan and P.E. thomas
Abstract


The efficiency of double antibody sandwitch (DAS) form of ELISA and the differences in transmission behavior of two closely related persistant phloem restricted circulatively aphid transmitted viruses , tomato yellow top virus and beet western yellow virus , was evaluated for the separation of single virion from mixed infection. Both viruses were successfully separated from doubly infected plants on account of extreme specificity, complete absence of heterologous reactivity and sensitivity of this technique.

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